Part:BBa_K1616023:Design

T7 promoter - HisTag- NanoLuc - T7 terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 117
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 20
  

Design Notes

Presence of XbaI site.

Source

NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.